作者
Benjamin F Bruner, Joel M Guthridge, Rufei Lu, Gabriel Vidal, Jennifer A Kelly, Julie M Robertson, Diane L Kamen, Gary S Gilkeson, Barbara R Neas, Morris Reichlin, R Hal Scofield, John B Harley, Judith A James
发表日期
2012/11
期刊
Arthritis & Rheumatism
卷号
64
期号
11
页码范围
3677-3686
出版商
Wiley Subscription Services, Inc., A Wiley Company
简介
Objective
Replacement of standard immunofluorescence methods with bead‐based assays for antinuclear antibody (ANA) testing is a new clinical option. The aim of this study was to evaluate a large, multiethnic cohort of patients with systemic lupus erytematosus (SLE), blood relatives, and unaffected control individuals for familial aggregation and subset clustering of autoantibodies by high‐throughput serum screening technology and traditional methods.
Methods
Serum samples (1,540 SLE patients, 1,154 unaffected relatives, and 906 healthy, population‐based controls) were analyzed for SLE autoantibodies using a bead‐based assay, indirect immunofluorescence (IIF), and immunodiffusion. Autoantibody prevalence, sensitivity for disease detection, clustering of autoantibodies, and associations between newer methods and standard immunodiffusion results were evaluated.
Results
The frequencies of ANAs …
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BF Bruner, JM Guthridge, R Lu, G Vidal, JA Kelly… - Arthritis & Rheumatism, 2012