作者
Judith A James, Joel M Guthridge, Hua Chen, Rufei Lu, Rebecka L Bourn, Krista Bean, Melissa E Munroe, Miles Smith, Eliza Chakravarty, Alan N Baer, Ghaith Noaiseh, Ann Parke, Karen Boyle, Lynette Keyes-Elstein, Andreea Coca, Tammy Utset, Mark C Genovese, Virginia Pascual, Paul J Utz, V Michael Holers, Kevin D Deane, Kathy L Sivils, Teresa Aberle, Daniel J Wallace, James McNamara, Nathalie Franchimont, E William St. Clair
发表日期
2020/4/1
期刊
Rheumatology
卷号
59
期号
4
页码范围
860-868
出版商
Oxford University Press
简介
Objective
To address heterogeneity complicating primary SS (pSS) clinical trials, research and care by characterizing and clustering patients by their molecular phenotypes.
Methods
pSS patients met American–European Consensus Group classification criteria and had at least one systemic manifestation and stimulated salivary flow of ⩾0.1 ml/min. Correlated transcriptional modules were derived from gene expression microarray data from blood (n = 47 with appropriate samples). Patients were clustered based on this molecular information using an unbiased random forest modelling approach. In addition, multiplex, bead-based assays and ELISAs were used to assess 30 serum cytokines, chemokines and soluble receptors. Eleven autoantibodies, including anti-Ro/SSA and anti-La/SSB, were measured by Bio-Rad Bioplex 2200.
Results
Transcriptional …
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JA James, JM Guthridge, H Chen, R Lu, RL Bourn… - Rheumatology, 2020