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P‐coumaric acid is a nonflavonoid phenolic acid and is a major constituent of the species Cynodon dactylon Linn. (Pers.). In this study isolation of P‐coumaric acid was achieved by preparative TLC and the compound thus isolated was characterised by UV, mass, and H¹ NMR spectral analysis. An isocratic RP‐HPLC method was developed for the estimation of P‐coumaric acid from methanolic extracts of durva grass. The chromatographic separations were achieved by RP‐C₁₈ column (250 mm × 4.6 mm, 5 μ), Shimadzu LC‐20AT Prominence liquid chromatograph, and a mobile phase composed of water : methanol : glacial acetic acid (65 : 34 : 1 v/v). The flow rate was 1.0 mL/min and the analyses of column effluents were performed using UV‐visible detector at 310 nm. Retention time of P‐coumaric acid was found to be 6.617 min. This method has obeyed linearity over the concentration range …