作者
Lingling Wu, Yuting Qin, Shiwei Xia, Min Dai, Xiao Han, Yanfang Wu, Xiaoyan Zhang, Jianyang Ma, Yan Wang, Yuanjia Tang, Zheng Liu, Wei Zhu, Bahija Jallal, Yihong Yao, Bo Qu, Nan Shen
发表日期
2016/5
期刊
Arthritis & rheumatology
卷号
68
期号
5
页码范围
1222-1232
简介
Objective
Type I interferon (IFN) signaling is regarded as a central pathogenic pathway in systemic lupus erythematosus (SLE). Specific inhibition of this pathway is a core area for the development of new therapies for SLE. This study was undertaken to clarify the pathogenic mechanism involved and to identify new therapeutic targets, using a high‐throughput screening platform to determine novel regulators that contribute to the overactivation of the type I IFN signaling pathway in SLE.
Methods
A high‐throughput IFN‐stimulated response element (ISRE)–luciferase assay was used to screen for candidate genes that regulate the IFN signaling pathway. Western blotting was used to confirm the regulatory function of CDK1. SYBR Green quantitative reverse transcriptase–polymerase chain reaction was used to detect the expression of individual IFN‐stimulated genes (ISGs). The differential expression of CDK1 and …
学术搜索中的文章
L Wu, Y Qin, S Xia, M Dai, X Han, Y Wu, X Zhang, J Ma… - Arthritis & rheumatology, 2016
L Wu, B Qu, Y Qin, N Shen - Arthritis & Rheumatology, 2015