作者
Dan Li, Yulan Zhao, Changxing Liu, Xiaona Chen, Yanting Qi, Yue Jiang, Chao Zou, Xiaolong Zhang, Shunying Liu, Xuejing Wang, Dan Zhao, Qiang Sun, Zhenbing Zeng, Andreas Dress, Marie C Lin, Hsiang-Fu Kung, Hallgeir Rui, Ling-Zhi Liu, Feng Mao, Bing-Hua Jiang, Lihui Lai
发表日期
2011/4/1
期刊
Clinical cancer research
卷号
17
期号
7
页码范围
1722-1730
出版商
American Association for Cancer Research
简介
Purpose: To investigate expression, regulation, potential role and targets of miR-195 and miR-497 in breast cancer.
Experimental Design: The expression patterns of miR-195 and miR-497 were initially examined in breast cancer tissues and cell lines by Northern blotting and quantitative real-time PCR. Combined bisulfite restriction analysis and bisulfite sequencing were carried out to study the DNA methylation status of miR-195 and miR-497 genes. Breast cancer cells stably expressing miR-195 and miR-497 were established to study their role and targets. Finally, normal, fibroadenoma and breast cancer tissues were employed to analyze the correlation between miR-195/497 levels and malignant stages of breast tumor tissues.
Results: MiR-195 and miR-497 were significantly downregulated in breast cancer. The methylation state of CpG islands upstream of the miR-195/497 gene …
学术搜索中的文章
D Li, Y Zhao, C Liu, X Chen, Y Qi, Y Jiang, C Zou… - Clinical cancer research, 2011