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Ectomycorrhizal fungi are important components of the carbon and nitrogen cycles in forest soils and improve the nutrient uptake of many plants. There have been few assessments of how soil fungal biomass is affected by climate; however, a good understanding of how soil mycelium is correlated with climate is essential to predict long-term responses to global warming. Soil extramatrical mycelia (EMM) of Lactarius vinosus, a highly valued and edible ectomycorrhizal species, was quantified by performing qPCR analyses of soil samples that were collected monthly from 28 long-term experimental plots. The belowground moisture and temperature of these plots were individually recorded every 2 h. L. vinosus soil EMM biomass fluctuated between 44 and 261 kg ha⁻¹, with the maximum biomass occurring during spring months and the minimum occurring during winter and summer months. Mean mycelial turnover …