作者
Nirmala Jagadish, Deepak Parashar, Namita Gupta, Sumit Agarwal, Vaishali Suri, Rajive Kumar, Vitusha Suri, Trilok Chand Sadasukhi, Anju Gupta, Abdul S Ansari, Nirmal Kumar Lohiya, Anil Suri
发表日期
2016/12
期刊
BMC cancer
卷号
16
页码范围
1-13
出版商
BioMed Central
简介
Background
Colorectal cancer (CRC) is the third leading cause of cancer related deaths worldwide both in men and women. Our recent studies have indicated an association of heat shock protein 70–2 (HSP70-2) with bladder urothelial carcinoma. In the present study, we investigated the association of HSP70-2 with various malignant properties of colorectal cancer cells and clinic-pathological features of CRC in clinical specimens.
Methods
HSP70-2 mRNA and protein was investigated expression by RT-PCR, immunohistochemistry, immunofluorescence, flow cytometry and Western blotting in CRC clinical specimens and COLO205 and HCT116 cell lines. Plasmid-based gene silencing approach was employed to study the association of HSP70-2 with various malignant properties of COLO205 and HCT116 cells in in vitro and with tumor progression in in …
学术搜索中的文章
N Jagadish, D Parashar, N Gupta, S Agarwal, V Suri… - BMC cancer, 2016
