作者
Michiel van Leeuwen, Nicolaas AA Buijs, André B Canelas, Arthur Oudshoorn, Joseph J Heijnen, Walter M van Gulik
发表日期
2009/4/15
期刊
Chemical engineering science
卷号
64
期号
8
页码范围
1877-1884
出版商
Pergamon
简介
We developed and applied a micropump for liquid flows of 0–150nL/min. The operating principle of this pump is based on the Hagen–Poiseuille law. The main advantages of this pump are that an accurate continuous flow rate can be achieved, it is cheap to manufacture, easy to parallelize and easy to integrate in microtiter plates. Experiments at different flow rates showed that the flow rate remained stable in time and the measured flow rates corresponded very well to the Hagen–Poiseuille law. The applicability of the pump for reproducible feeding of microbioreactors was shown in a fed-batch system with a simple chemical reaction. Thereby the Hagen–Poiseuille pump was connected to a microbioreactor with a working volume of 100μL. The microreactor was filled with a H2SO4 solution which was fed with a Na2CO3 solution at a constant rate, while measuring the CO2 release on-line. This resulted in a stable CO …
引用总数
200920102011201220132014201520162017201820191212112
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M van Leeuwen, NAA Buijs, AB Canelas, A Oudshoorn… - Chemical engineering science, 2009