作者
Jarne Postmus, André B Canelas, Jildau Bouwman, Barbara M Bakker, Walter van Gulik, M Joost Teixeira de Mattos, Stanley Brul, Gertien J Smits
发表日期
2008/8/29
期刊
Journal of Biological Chemistry
卷号
283
期号
35
页码范围
23524-23532
出版商
Elsevier
简介
A major challenge in systems biology lies in the integration of processes occurring at different levels, such as transcription, translation, and metabolism, to understand the functioning of a living cell in its environment. We studied the high temperature-induced glycolytic flux increase in Saccharomyces cerevisiae and investigated the regulatory mechanisms underlying this increase. We used glucose-limited chemostat cultures to separate regulatory effects of temperature from effects on growth rate. Growth at increased temperature (38 °C versus 30 °C) resulted in a strongly increased glycolytic flux, accompanied by a switch from respiration to a partially fermentative metabolism. We observed an increased flux through all enzymes, ranging from 5- to 10-fold. We quantified the contributions of direct temperature effects on enzyme activities, the gene expression cascade and shifts in the metabolic network, to the increased …
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J Postmus, AB Canelas, J Bouwman, BM Bakker… - Journal of Biological Chemistry, 2008