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This study examined the practice of breast self‐examination and knowledge of breast cancer among female university students in Korea. This was a cross‐sectional descriptive study. The participants were 2186 female university students in Korea. The Breast Cancer and Heredity Knowledge Scale was used. The collected data were analyzed through descriptive statistics, χ²‐test, t‐test, ANOVA, and logistic regression. Twenty‐seven percent of students reported engaging in breast self‐examination. The participants displayed a medium‐level score (total score: 5.33 ± 2.70, range: 0–11) on knowledge about breast cancer. Predictors for breast self‐examination were age (odds ratio = 1.15, P < 0.001), major (odds ratio = 1.80, P < 0.001), and knowledge of breast cancer (odds ratio = 1.16, P < 0.001). This study was the sole nationwide‐scale research that examined the practice of breast self …