作者
Geoffrey R Oxnard, Cloud P Paweletz, Yanan Kuang, Stacy L Mach, Allison O'Connell, Melissa M Messineo, Jason J Luke, Mohit Butaney, Paul Kirschmeier, David M Jackman, Pasi A Jänne
发表日期
2014/3/15
期刊
Clinical cancer research
卷号
20
期号
6
页码范围
1698-1705
出版商
American Association for Cancer Research
简介
Purpose: Tumor genotyping using cell-free plasma DNA (cfDNA) has the potential to allow noninvasive assessment of tumor biology, yet many existing assays are cumbersome and vulnerable to false-positive results. We sought to determine whether droplet digital PCR (ddPCR) of cfDNA would allow highly specific and quantitative assessment of tumor genotype.
Experimental Design: ddPCR assays for EGFR, KRAS, and BRAF mutations were developed using plasma collected from patients with advanced lung cancer or melanoma of a known tumor genotype. Sensitivity and specificity were determined using cancers with nonoverlapping genotypes as positive and negative controls. Serial assessment of response and resistance was studied in patients with EGFR-mutant lung cancer on a prospective trial of erlotinib.
Results: We identified a reference range for EGFR L858R and exon …
学术搜索中的文章
GR Oxnard, CP Paweletz, Y Kuang, SL Mach… - Clinical cancer research, 2014