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In recent years, the biosynthesized of metallic nanoparticles has been rapidly growing due to their environmentally synthesis and widespread applications in science and industry. In this study, we have reported the green synthesis of NiONPs using an aqueous extract of Trigonella subenervis (NiONPs@TS). The nanoparticles were characterized by Fourier transform infrared spectroscopy (FT‐IR), X‐ray diffraction (XRD), field emission scanning electron microscopy (FE‐SEM), and energy‐dispersive X‐ray spectroscopy (EDS). To find various applications for NiONPs@TS, the nanoparticles (NPs) were used as a catalyst for synthesizing some benzimidazole derivatives. The electrochemical behavior and antibacterial activity of NiONPs@TS were also evaluated. FE‐SEM images exhibited a uniform spherical morphology in size of 28.21 nm for the biosynthesized nanoparticles. According to the XRD analysis, 26.43 …