作者
Gary Reynolds, Peter Vegh, James Fletcher, Elizabeth FM Poyner, Emily Stephenson, Issac Goh, Rachel A Botting, Ni Huang, Bayanne Olabi, Anna Dubois, David Dixon, Kile Green, Daniel Maunder, Justin Engelbert, Mirjana Efremova, Krzysztof Polański, Laura Jardine, Claire Jones, Thomas Ness, Dave Horsfall, Jim McGrath, Christopher Carey, Dorin-Mirel Popescu, Simone Webb, Xiao-nong Wang, Ben Sayer, Jong-Eun Park, Victor A Negri, Daria Belokhvostova, Magnus Lynch, David McDonald, Andrew Filby, Tzachi Hagai, Kerstin B Meyer, Akhtar Husain, Jonathan Coxhead, Roser Vento-Tormo, Sam Behjati, Steven Lisgo, Alexandra-Chloé Villani, Jaume Bacardit, Phil Jones, Edel A O’Toole, Graham S Ogg, Neil Rajan, Nick J Reynolds, Sarah A Teichmann, Fiona Watt, Muzlifah Haniffa
发表日期
2020/11/5
期刊
BioRxiv
页码范围
2020.11. 05.369363
出版商
Cold Spring Harbor Laboratory
简介
The human skin confers biophysical and immunological protection through a complex cellular network that is established early in development. We profiled ~500,000 single cells using RNA-sequencing from healthy adult and developing skin, and skin from patients with atopic dermatitis and psoriasis. Our findings reveal a predominance of innate lymphoid cells and macrophages in developing skin in contrast to T cells and migratory dendritic cells in adult skin. We demonstrate dual keratinocyte differentiation trajectories and activated cellular circuits comprising vascular endothelial cells mediating immune cell trafficking, disease-specific clonally expanded IL13/IL22 and IL17A/F-expressing lymphocytes, epidermal IL23-expressing dendritic cells and inflammatory keratinocytes in disease. Our findings provide key insights into the dynamic cellular landscape of human skin in health and disease.
One Sentence Summary
Single cell atlas of human skin reveals cell circuits which are quantitatively and qualitatively reconfigured in inflammatory skin disease.
学术搜索中的文章
G Reynolds, P Vegh, J Fletcher, EFM Poyner… - BioRxiv, 2020