作者
Peter Vegh, David A Magee, Nicolas C Nalpas, Kenneth Bryan, Matthew S McCabe, John A Browne, Kevin M Conlon, Stephen V Gordon, Daniel G Bradley, David E MacHugh, David J Lynn
发表日期
2015/1/1
期刊
Tuberculosis
卷号
95
期号
1
页码范围
60-67
出版商
Churchill Livingstone
简介
Mycobacterium bovis, the causative agent of bovine tuberculosis, a major problem for global agriculture, spreads via an airborne route and is taken up by alveolar macrophages (AM) in the lung. Here, we describe the first next-generation sequencing (RNA-seq) approach to temporally profile miRNA expression in primary bovine AMs post-infection with M. bovis. One, six, and forty miRNAs were identified as significantly differentially expressed at 2, 24 and 48 h post-infection, respectively. The differential expression of three miRNAs (bta-miR-142-5p, bta-miR-146a, and bta-miR-423-3p) was confirmed by RT-qPCR. Pathway analysis of the predicted mRNA targets of differentially expressed miRNAs suggests that these miRNAs preferentially target several pathways that are functionally relevant for mycobacterial pathogenesis, including endocytosis and lysosome trafficking, IL-1 signalling and the TGF-β pathway. Over …
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P Vegh, DA Magee, NC Nalpas, K Bryan, MS McCabe… - Tuberculosis, 2015