作者
Peter Vegh, Amir BK Foroushani, David A Magee, Matthew S McCabe, John A Browne, Nicolas C Nalpas, Kevin M Conlon, Stephen V Gordon, Daniel G Bradley, David E MacHugh, David J Lynn
发表日期
2013/10/1
期刊
Veterinary Immunology and Immunopathology
卷号
155
期号
4
页码范围
238-244
出版商
Elsevier
简介
MicroRNAs (miRNAs) are important regulators of gene expression and are known to play a key role in regulating both adaptive and innate immunity. Bovine alveolar macrophages (BAMs) help maintain lung homeostasis and constitute the front line of host defense against several infectious respiratory diseases, such as bovine tuberculosis. Little is known, however, about the role miRNAs play in these cells. In this study, we used a high-throughput sequencing approach, RNA-seq, to determine the expression levels of known and novel miRNAs in unchallenged BAMs isolated from lung lavages of eight different healthy Holstein–Friesian male calves. Approximately 80 million sequence reads were generated from eight BAM miRNA Illumina sequencing libraries, and 80 miRNAs were identified as being expressed in BAMs at a threshold of at least 100 reads per million (RPM). The expression levels of miRNAs varied …
学术搜索中的文章
P Vegh, ABK Foroushani, DA Magee, MS McCabe… - Veterinary Immunology and Immunopathology, 2013