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Polluted soil is a reservoir of tolerant fungiFungi, which can be isolated to employ them in mycoremediation. Tolerance is a relevant characteristic for selecting fungal strains capable of growing in the presence of heavy metalsHeavy metals. A very interesting and effective strategy for the isolation of tolerant fungiFungi is the spread plateSpread plate method. Tolerance can be measured by plaque screeningScreenings technique, and fungal development can be understood by predictive mycologyPredictive mycology. Therefore, tolerant isolates have a promising ability to be applied in the bioremediationBioremediation of heavy metalsHeavy metals.