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Biodeterioration is a topic of ever-growing concern and is particularly relevant in the context of cultural heritage conservation, since artworks and monuments provide diversified ecological niches for microorganism colonization. Despite all the gathered knowledge in recent years, current established norms and accepted contamination thresholds have a prominent focus on human health and air quality preservation. Nonetheless they still are not enough or are not adequately applied for cultural heritage preservation. In the light of this study within a very important Museum from Coimbra (Portugal), the current knowledge and accepted norms are discussed. Despite the meticulous control of environmental parameters inside this art repository, the presence of fungal colonies was unexpectedly detected on wooden sculptures and paintings that were deposited inside a custom-built room. Contaminated art objects were …