作者
Limin Shi, Qinlu Lin, Xinhua Li, Ying Nie, Shuguo Sun, Xiyun Deng, Long Wang, Jun Lu, Yiping Tang, Feijun Luo
发表日期
2017/9
期刊
Molecular Nutrition & Food Research
卷号
61
期号
9
页码范围
1601013
简介
Scope
In this study, the anti‐inflammatory effects and the molecular mechanism of alliin were analyzed in dextran sulfate sodium (DSS)‐induced colitis mice and lipopolysaccharide‐stimulated RAW264.7 cell model.
Methods
The phenotype of mice was recorded in the DSS‐induced and/or alliin (500 mg/kg) groups. Histopathological alterations were analyzed by H&E staining. MPO and MDA of colon tissues were measured. The mRNA expression levels of inflammatory factors were determined by qRT‐PCR, and protein expressions of inflammatory factors or activation of kinases were determined by Western blotting.
Results
Oral administration of alliin significantly inhibited the decrease of body weight, improved the DAI and decreased the infiltration of inflammatory cells in colonic tissues. The content of NO, MDA, and MPO, the expression of iNOS and inflammatory factors as well as MAPK and the …
学术搜索中的文章
L Shi, Q Lin, X Li, Y Nie, S Sun, X Deng, L Wang, J Lu… - Molecular Nutrition & Food Research, 2017