作者
Yun Zheng, Yong-Fang Li, Ramanjulu Sunkar, Weixiong Zhang
发表日期
2012/2/1
期刊
Nucleic acids research
卷号
40
期号
4
页码范围
e28-e28
出版商
Oxford University Press
简介
In plants, microRNAs (miRNAs) regulate their mRNA targets by precisely guiding cleavages between the 10th and 11th nucleotides in the complementary regions. High-throughput sequencing-based methods, such as PARE or degradome profiling coupled with a computational analysis of the sequencing data, have recently been developed for identifying miRNA targets on a genome-wide scale. The existing algorithms limit the number of mismatches between a miRNA and its targets and strictly do not allow a mismatch or G:U Wobble pair at the position 10 or 11. However, evidences from recent studies suggest that cleavable targets with more mismatches exist indicating that a relaxed criterion can find additional miRNA targets. In order to identify targets including the ones with weak complementarities from degradome data, we developed a computational method called SeqTar that allows more …
学术搜索中的文章
Y Zheng, YF Li, R Sunkar, W Zhang - Nucleic acids research, 2012