作者
Lisa J Hill, Ben Mead, Chloe N Thomas, Simon Foale, Elena Feinstein, Martin Berry, Richard J Blanch, Zubair Ahmed, Ann Logan
发表日期
2018
期刊
Molecular vision
卷号
24
页码范围
712
出版商
Emory University
简介
Purpose
Elevations in intraocular pressure (IOP) are associated with the development of glaucoma and loss of sight. High transforming growth factor-β (TGF-β) 1 levels in the eye’s anterior chamber can lead to dysfunctional contractions through RhoA signaling in trabecular meshwork (TM) cells and IOP spikes. Sustained high TGF-β levels leads to TM fibrosis and sustained increases in IOP. We investigated whether inhibiting RhoA, using a siRNA-mediated RhoA (siRhoA), controls IOP by altering TM expression of fibrosis and contractility-related proteins in a rodent model of glaucoma.
Methods
TGF-β was injected intracamerally twice a week into adult Sprague Dawley rats, and IOP was recorded with tonometry. Animals were euthanized on day 7 and 35 with TM expression of fibrosis and contractility-related proteins, as well as survival of retinal ganglion cells (RGCs) assessed with immunohistochemistry. siRNA …
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LJ Hill, B Mead, CN Thomas, S Foale, E Feinstein… - Molecular vision, 2018