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We collected ticks from camels in 4 regions of southern Algeria (El Oued, Bechar, Ghardia, and Adrar) from February to October in 2008 and in April of 2011. A total of 307 ticks representing multiple species (including Hyalomma dromedarii, H. marginatum rufipes, H. impeltatum, and H. impressum), was tested for the presence of spotted fever group rickettsia DNA using gltA real-time quantitative PCR (qPCR). The presence of Rickettsia aeschlimannii was confirmed with a new qPCR using species-specific primers and Taqman probes based on the sca2 genes. The R. aeschlimannii sequence was further confirmed by detecting the gltA and outer membrane protein (ompA) genes in H. m. rufipes, H. impeltatum, and H. dromedarii ticks. These findings represent the first report of the detection of R. aeschlimannii in ticks collected from camels from southern Algeria.