作者
Meriem Bellabidi, Mohammed Hocine Benaissa, Samia Bissati-Bouafia, Zoubir Harrat, Karima Brahmi, Tahar Kernif
发表日期
2020/6/1
期刊
Acta Tropica
卷号
206
页码范围
105443
出版商
Elsevier
简介
Q fever is a widespread zoonotic disease caused by Coxiella burnetii that most commonly infects not only a variety of mammals but also arthropods and in particularly ticks. The aim of this study was to detect C. burnetii infection in camels including ixodid ticks using serological and molecular assays. Between July 2018 to June 2019, blood samples from 184 male and female camels (Camelus dromedarius) were collected from 3 regions of South–East Algeria and serum samples were tested for antibodies against Coxiella burnetii using indirect enzyme-linked immunosorbent assay (ELISA) kit. The positive sera and a total of 60 ticks were tested by quantitative PCR (qPCR) for detection of C. burnetii with primers and probes specific to the transposon-like repetitive region (IS1111 gene). Positive samples were genotyped by amplification and sequencing of partial sequences based on the IS1111 gene.
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M Bellabidi, MH Benaissa, S Bissati-Bouafia, Z Harrat… - Acta Tropica, 2020