作者
Heather L Greenstone, John D Nieland, Karin E De Visser, Marloes LH De Bruijn, Reinhard Kirnbauer, Richard BS Roden, Douglas R Lowy, W Martin Kast, John T Schiller
发表日期
1998/2/17
期刊
Proceedings of the National Academy of Sciences
卷号
95
期号
4
页码范围
1800-1805
出版商
The National Academy of Sciences
简介
Papillomavirus-like particles (VLPs) are a promising prophylactic vaccine candidate to prevent human papillomavirus (HPV) infections and associated epithelial neoplasia. However, they are unlikely to have therapeutic effects because the virion capsid proteins are not detected in the proliferating cells of the infected epithelia or in cervical carcinomas. To increase the number of viral antigen targets for cell-mediated immune responses in a VLP-based vaccine, we have generated stable chimeric VLPs consisting of the L1 major capsid protein plus the entire E7 (11 kDa) or E2 (43 kDa) nonstructural papillomavirus protein fused to the L2 minor capsid protein. The chimeric VLPs are indistinguishable from the parental VLPs in their morphology and in their ability to agglutinate erythrocytes and elicit high titers of neutralizing antibodies. Protection from tumor challenge was tested in C57BL/6 mice by using the tumor cell …
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