作者
Elena Baixeras, B Huard, Cet al Miossec, S Jitsukawa, M Martin, T Hercend, C Auffray, F Triebel, D Piatier-Tonneau
发表日期
1992/8/1
期刊
The Journal of experimental medicine
卷号
176
期号
2
页码范围
327-337
简介
The lymphocyte activation gene 3 (LAG-3), expressed in human activated T and natural killer (NK) cells, is closely related to CD4 at the gene and protein levels. We report here the initial characterization of the LAG-3-encoded protein. We have generated two monoclonal antibodies after immunization of mice with a 30-amino acid peptide that corresponds to an exposed extra loop region present in the LAG-3 immunoglobulin-like first domain. The reactivity of these reagents is directed against LAG-3 since they recognize both membrane-expressed and soluble recombinant LAG-3 molecules produced in a baculovirus expression system. The two antibodies are likely to react with the same or closely related epitope (termed LAG-3.1) exposed on the LAG-3 first domain extra loop, as assessed in competition experiments on LAG-3-expressing activated lymphocytes. Cellular distribution analysis indicated that the LAG-3.1 …
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E Baixeras, B Huard, C Miossec, S Jitsukawa, M Martin… - The Journal of experimental medicine, 1992