作者
Sadi Loai, Inga Haedicke, Zahra Mirzaei, Craig A Simmons, Xiao‐an Zhang, Hai Ling Cheng
发表日期
2016/12
期刊
Journal of Magnetic Resonance Imaging
卷号
44
期号
6
页码范围
1456-1463
简介
Purpose
To investigate the feasibility of high‐sensitivity cellular MRI of embryonic stem (ES) cells using a novel cell permeable and cell retentive T1 contrast agent.
Materials and Methods
Mouse ES cells were labeled with a novel manganese porphyrin contrast agent, MnAMP, at 0.1 mM over 2 to 24 h and retained in contrast‐free medium for up to 24 h postlabeling. MRI was performed on a 3 Tesla clinical scanner; T1 and T2 relaxation times were measured. Quantification of manganese content was performed using atomic absorption spectroscopy. Viability and proliferation assays were done for the longest labeling interval. Differentiation capacity was assessed using the hanging drop method to direct differentiation toward cardiomyocytes.
Results
MnAMP‐labeled ES cells exhibited over a fourfold decrease in T1 compared with unlabeled cells, and maintained up to a threefold decrease 24 h postlabeling …
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S Loai, I Haedicke, Z Mirzaei, CA Simmons, X Zhang… - Journal of Magnetic Resonance Imaging, 2016