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Generation status is one indicator of acculturation that may affect the chronic pain experience for Mexican Americans (MAs). Using data from the 1999-2004 National Health and Nutrition Examination Survey (NHANES), prevalence of chronic pain, healthcare insurance coverage, and analgesic medication use were examined among first-, second-, and≥ third-generation MAs. Logistic regression models adjusted for age, sex, and sampling design examined generation status differences in:(1) chronic pain (≥ 3 months) prevalence among all MA respondents (n= 3,353) and (2) location of pain, healthcare insurance coverage, and analgesic medication use among MA respondents with chronic pain (n= 331). Across all respondents, second-and≥ third-generation MAs had higher odds of reporting any chronic pain than first-generation MAs (OR= 1.92 [1.41-2.62]; OR= 2.50 [1.69-3.69], ps<. 0001, respectively …