作者
José M Rodrigo‐Muñoz, José A Cañas, Beatriz Sastre, Natalia Rego, Gonzalo Greif, Manuel Rial, Pablo Mínguez, Ignacio Mahíllo‐Fernández, Mar Fernández‐Nieto, Inés Mora, Pilar Barranco, Santiago Quirce, Joaquin Sastre, Victoria Del Pozo
发表日期
2019/3
期刊
Allergy
卷号
74
期号
3
页码范围
507-517
简介
Background
Asthma is a syndrome characterized by airway inflammation and obstruction. Due to its heterogeneity, the difficulties in asthma diagnosis and treatment make the discovery of new biomarkers a focus of research. So, we determined the differential miRNA expression of eosinophils between healthy and asthmatic patients and to establish a differentially expressed miRNA profile detectable in sera for use as biomarker.
Methods
MicroRNAs from peripheral eosinophils from healthy and asthmatic subjects were isolated and analyzed by next‐generation sequencing and confirmed by quantitative PCR in 29 asthmatics and 10 healthy individuals. The levels of serum miRNAs were performed by quantitative PCR in 138 asthmatics and 39 healthy subjects. Regression analysis and Random Forest models were performed.
Results
We found a set of miRNAs whose expression differs between eosinophils from …
学术搜索中的文章
JM Rodrigo‐Muñoz, JA Cañas, B Sastre, N Rego… - Allergy, 2019