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Background Platelets play a pivotal role in wound healing. Their beneficial effect is attributed to the release of bioactive substances, although the involved mechanisms are mostly unknown.

Objectives To investigate mechanisms underlying platelet‐induced wound healing using HaCaT keratinocytes, representing an in vitro model of proliferating and migrating keratinocytes.

Methods Cells were exposed to platelet lysate (PL) purified from whole blood samples. Cell metabolism and proliferation were assessed using MTS and crystal violet assays, respectively, wound healing was assessed by scratch wound assay and cell migration by transwell assay. Extracellular signal‐regulated kinase (ERK) 1/2 and p38 activations were studied using Western immunoblotting and intracellular Ca²⁺ dynamics by confocal imaging.

Results Wound closure rates showed a significant …