作者
Dharma Koirala, Shreeti Pradhan, Bijaya Pant
发表日期
2013/9/5
期刊
Scientific world
卷号
11
期号
11
页码范围
97-100
简介
Coelogyne fuscescens Lindl. is one of the commercially threatened epiphytic orchid of Nepal. It has reported to have medicinal properties as its pseudobulb is used to reduce the abdominal pain. Germination of the orchid seeds in nature is difficult without the symbiotic association of the Mycorrhizal fungi, Rhizoctonia species. Therefore, the present research was focused to study the effect of growth hormones on asymbiotic seed germination and their subsequent development into plantlets. Immature seeds of this orchid species cultured in MS media supplemented with different concentrations of 6–Benzylaminopurine, BAP (0.5 mg/l-2mg/l) and α-Napthalene Acetic Acid, NAA (0.5 mg/l), singly and in combination showed various responses on germination and seedling development. MS medium supplemented with BAP (1.0 mg/l) plus NAA (0.5 mg/l) was found to be the most favourable condition for the early seed germination and protocorm development while medium supplemented with NAA (0.5 mg/l) was found to be favourable for their further differentiation into plantlets.
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