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The distribution of organic C and trace elements (Cd, Cu, and Ni) between truly dissolved and colloidal phases was determined in May 1992, in order to better understand their behavior during estuarine mixing in the Rhône delta (France). Colloidal material (10⁴ Daltons-0.4 μm) was separated with an ultra-clean cross-flow filtration system. A considerable part of the organic C and trace metals studied, used to be considered to occur in the so-called “dissolved” pool, is associated with colloidal material. The significant correlation between colloidal organic C and colloidal Cu suggests an important involvement of colloidal organic material in the transport of this element. The correlation between Ni, Cd and organic matter mainly occurs in the truly dissolved fraction, suggesting that the truly dissolved organic C is important for these two elements. Truly dissolved and colloidal organic C and trace-metal fluxes transported …