作者
Marc Clos-Garcia, Naiara Andrés-Marin, Gorka Fernández-Eulate, Leticia Abecia, José L Lavín, Sebastiaan van Liempd, Diana Cabrera, Félix Royo, Alejandro Valero, Nerea Errazquin, María Cristina Gómez Vega, Leila Govillard, Michael R Tackett, Genesis Tejada, Esperanza Gónzalez, Juan Anguita, Luis Bujanda, Ana María Callejo Orcasitas, Ana M Aransay, Olga Maíz, Adolfo López de Munain, Juan Manuel Falcón-Pérez
发表日期
2019/8/1
期刊
EBioMedicine
卷号
46
页码范围
499-511
出版商
Elsevier
简介
Background
Fibromyalgia is a complex, relatively unknown disease characterised by chronic, widespread musculoskeletal pain. The gut-brain axis connects the gut microbiome with the brain through the enteric nervous system (ENS); its disruption has been associated with psychiatric and gastrointestinal disorders. To gain an insight into the pathogenesis of fibromyalgia and identify diagnostic biomarkers, we combined different omics techniques to analyse microbiome and serum composition.
Methods
We collected faeces and blood samples to study the microbiome, the serum metabolome and circulating cytokines and miRNAs from a cohort of 105 fibromyalgia patients and 54 age- and environment-matched healthy individuals. We sequenced the V3 and V4 regions of the 16S rDNA gene from faeces samples. UPLC-MS metabolomics and custom multiplex cytokine and miRNA analysis (FirePlex™ technology) were …
学术搜索中的文章
M Clos-Garcia, N Andrés-Marin, G Fernández-Eulate… - EBioMedicine, 2019