作者
Pamela J Plant, Frank Lafont, Sandra Lecat, Paul Verkade, Kai Simons, Daniela Rotin
发表日期
2000/6/26
期刊
The Journal of cell biology
卷号
149
期号
7
页码范围
1473-1484
出版商
The Rockefeller University Press
简介
Nedd4 is a ubiquitin protein ligase (E3) containing a C2 domain, three or four WW domains, and a ubiquitin ligase HECT domain. We have shown previously that the C2 domain of Nedd4 is responsible for its Ca2+-dependent targeting to the plasma membrane, particularly the apical region of epithelial MDCK cells. To investigate this apical preference, we searched for Nedd4-C2 domain-interacting proteins that might be involved in targeting Nedd4 to the apical surface. Using immobilized Nedd4-C2 domain to trap interacting proteins from MDCK cell lysate, we isolated, in the presence of Ca2+, a ∼35–40-kD protein that we identified as annexin XIII using mass spectrometry. Annexin XIII has two known isoforms, a and b, that are apically localized, although XIIIa is also found in the basolateral compartment. In vitro binding and coprecipitation experiments showed that the Nedd4-C2 domain interacts with both annexin …
引用总数
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Apical membrane targeting of Nedd4 is mediated by an association of its C2 domain with annexin XIIIb
PJ Plant, F Lafont, S Lecat, P Verkade, K Simons… - The Journal of cell biology, 2000