作者
Khirod K Sahoo, Amit K Tripathi, Ashwani Pareek, Sudhir K Sopory, Sneh L Singla-Pareek
发表日期
2011/12/30
期刊
Plant methods
卷号
7
期号
1
页码范围
49
出版商
BioMed Central
简介
Background
Rice genome sequencing projects have generated remarkable amount of information about genes and genome architecture having tremendous potential to be utilized in both basic and applied research. Success in transgenics is paving the way for preparing a road map of functional genomics which is expected to correlate action of a gene to a trait in cellular and organismal context. However, the lack of a simple and efficient method for transformation and regeneration is a major constraint for such studies in this important cereal crop.
Results
In the present study, we have developed an easy, rapid and highly efficient transformation and regeneration protocol using mature seeds as explants and found its successful applicability to a choice of elite indica rice genotypes. We have optimized various steps of transformation and standardized …
学术搜索中的文章
KK Sahoo, AK Tripathi, A Pareek, SK Sopory… - Plant methods, 2011