作者
Martin Guilliams, Charles-Antoine Dutertre, Charlotte L Scott, Naomi McGovern, Dorine Sichien, Svetoslav Chakarov, Sofie Van Gassen, Jinmiao Chen, Michael Poidinger, Sofie De Prijck, Simon J Tavernier, Ivy Low, Sergio Erdal Irac, Citra Nurfarah Mattar, Hermi Rizal Sumatoh, Gillian Hui Ling Low, Tam John Kit Chung, Dedrick Kok Hong Chan, Ker Kan Tan, Tony Lim Kiat Hon, Even Fossum, Bjarne Bogen, Mahesh Choolani, Jerry Kok Yen Chan, Anis Larbi, Hervé Luche, Sandrine Henri, Yvan Saeys, Evan William Newell, Bart N Lambrecht, Bernard Malissen, Florent Ginhoux
发表日期
2016/9/20
期刊
Immunity
卷号
45
期号
3
页码范围
669-684
出版商
Elsevier
简介
Dendritic cells (DCs) are professional antigen-presenting cells that hold great therapeutic potential. Multiple DC subsets have been described, and it remains challenging to align them across tissues and species to analyze their function in the absence of macrophage contamination. Here, we provide and validate a universal toolbox for the automated identification of DCs through unsupervised analysis of conventional flow cytometry and mass cytometry data obtained from multiple mouse, macaque, and human tissues. The use of a minimal set of lineage-imprinted markers was sufficient to subdivide DCs into conventional type 1 (cDC1s), conventional type 2 (cDC2s), and plasmacytoid DCs (pDCs) across tissues and species. This way, a large number of additional markers can still be used to further characterize the heterogeneity of DCs across tissues and during inflammation. This framework represents the way …
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M Guilliams, CA Dutertre, CL Scott, N McGovern… - Immunity, 2016