作者
David B Neale, Jill L Wegrzyn, Kristian A Stevens, Aleksey V Zimin, Daniela Puiu, Marc W Crepeau, Charis Cardeno, Maxim Koriabine, Ann E Holtz-Morris, John D Liechty, Pedro J Martínez-García, Hans A Vasquez-Gross, Brian Y Lin, Jacob J Zieve, William M Dougherty, Sara Fuentes-Soriano, Le-Shin Wu, Don Gilbert, Guillaume Marçais, Michael Roberts, Carson Holt, Mark Yandell, John M Davis, Katherine E Smith, Jeffrey FD Dean, W Walter Lorenz, Ross W Whetten, Ronald Sederoff, Nicholas Wheeler, Patrick E McGuire, Doreen Main, Carol A Loopstra, Keithanne Mockaitis, Pieter J deJong, James A Yorke, Steven L Salzberg, Charles H Langley
发表日期
2014/3
期刊
Genome biology
卷号
15
页码范围
1-13
出版商
BioMed Central
简介
Background
The size and complexity of conifer genomes has, until now, prevented full genome sequencing and assembly. The large research community and economic importance of loblolly pine, Pinus taeda L., made it an early candidate for reference sequence determination.
Results
We develop a novel strategy to sequence the genome of loblolly pine that combines unique aspects of pine reproductive biology and genome assembly methodology. We use a whole genome shotgun approach relying primarily on next generation sequence generated from a single haploid seed megagametophyte from a loblolly pine tree, 20-1010, that has been used in industrial forest tree breeding. The resulting sequence and assembly was used to generate a draft genome spanning 23.2 Gbp and containing 20.1 Gbp with an N50 scaffold size of 66.9 kbp, making it a …
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DB Neale, JL Wegrzyn, KA Stevens, AV Zimin, D Puiu… - Genome biology, 2014