作者
Magdalena Klanova, Ladislav Andera, Jan Brazina, Jan Svadlenka, Simona Benesova, Jan Soukup, Dana Prukova, Dana Vejmelkova, Radek Jaksa, Karel Helman, Petra Vockova, Lucie Lateckova, Jan Molinsky, Bokang Calvin Lenyeletse Maswabi, Mahmudul Alam, Roman Kodet, Robert Pytlik, Marek Trneny, Pavel Klener
发表日期
2016/3/1
期刊
Clinical Cancer Research
卷号
22
期号
5
页码范围
1138-1149
出版商
American Association for Cancer Research
简介
Purpose: To investigate the roles of BCL2, MCL1, and BCL-XL in the survival of diffuse large B-cell lymphoma (DLBCL).
Experimental designs: Immunohistochemical analysis of 105 primary DLBCL samples, and Western blot analysis of 18 DLBCL cell lines for the expression of BCL2, MCL1, and BCL-XL. Pharmacologic targeting of BCL2, MCL1, and BCL-XL with ABT-199, homoharringtonine (HHT), and ABT-737. Analysis of DLBCL clones with manipulated expressions of BCL2, MCL1, and BCL-XL. Immunoprecipitation of MCL1 complexes in selected DLBCL cell lines. Experimental therapy aimed at inhibition of BCL2 and MCL1 using ABT-199 and HHT, single agent, or in combination, in vitro and in vivo on primary cell-based murine xenograft models of DLBCL.
Results: By the pharmacologic targeting of BCL2, MCL1, and BCL-XL, we demonstrated that DLBCL can be divided into …
学术搜索中的文章
M Klanova, L Andera, J Brazina, J Svadlenka… - Clinical Cancer Research, 2016