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Objective and design

The clearance receptor for natriuretic peptides (NPRC), a candidate gene for essential hypertension, is highly expressed in adipose tissue, where is nutritionally regulated. The objectives of the present study were to sequence the human 5'flanking regulatory region of NPRC, to identify allelic variants and their frequencies, and to study the genotype/phenotype correlation in hypertensive patients.

Methods and results

Using polymerase chain reaction (PCR) and direct automated sequencing, a biallelic (A/C) polymorphism was detected at position− 55 in a conserved promoter element named P1. The novel C (− 55) variant makes the promoter sequence identical to the mouse gene and introduces a second HgaI site in the amplified DNA, allowing the genotyping of a large number of subjects. In a random sample of 232 white Caucasians the C (− 55) allele was more commonly found (81.7% of all …