作者
Klaus Schlaeppi, S Franz Bender, Fabio Mascher, Giancarlo Russo, Andrea Patrignani, Tessa Camenzind, Stefan Hempel, Matthias C Rillig, Marcel GA van der Heijden
发表日期
2016/11
期刊
New Phytologist
卷号
212
期号
3
页码范围
780-791
简介
- Community analyses of arbuscular mycorrhizal fungi (AMF) using ribosomal small subunit (SSU) or internal transcribed spacer (ITS) DNA sequences often suffer from low resolution or coverage. We developed a novel sequencing based approach for a highly resolving and specific profiling of AMF communities.
- We took advantage of previously established AMF‐specific PCR primers that amplify a c. 1.5‐kb long fragment covering parts of SSU, ITS and parts of the large ribosomal subunit (LSU), and we sequenced the resulting amplicons with single molecule real‐time (SMRT) sequencing.
- The method was applicable to soil and root samples, detected all major AMF families and successfully discriminated closely related AMF species, which would not be discernible using SSU sequences. In inoculation tests we could trace the introduced AMF inoculum at the molecular level. One of the introduced strains almost …
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K Schlaeppi, SF Bender, F Mascher, G Russo… - New Phytologist, 2016