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The aim of the present study was to investigate the impact of a natural citrus flavonoglycoside hesperidin on the human mammary carcinoma cell line (MCF-7) and its possible role in the induction of apoptosis. Human breast cancer cells were treated with different concentrations of hesperidin and its cytotoxicity effect was measured by MTT method and lactate dehydrogenase leakage assays. Apoptosis was analyzed by light microscopic studies and DNA fragmentation. The mechanism of apoptosis was studied with the expression of p53 and caspase-3 proteins. Glutathione content was also measured in MCF-7 cells after hesperidin treatment. The results revealed that hesperidin significantly inhibited the proliferation of MCF-7 cells in a concentration-dependent manner. Apoptotic morphology was observed in MCF-7 cells treated with hesperidin at the concentration of 60 and 80 μM. DNA fragmentation, accumulation …