作者
Manar M Farouk, Amal El-Molla, Fayez A Salib, Yousef A Soliman
发表日期
2022/3/10
卷号
54
期号
1
页码范围
381-389
简介
A cross sectional survey study aimed to: (1) Identify the management factors associated with the occurrence of salmonellosis in some diarrheic sheep and goats belonging to mixed reared flocks in Giza governorate, Egypt, (2) Investigate the presence of enterotoxin(stn) gene in the recovered Salmonella spp. strains, and (3) Build a phylogenetic tree for the partial codon sequence of stn gene of some recovered strains in order to provide a scientific basis for the implementation of practical preventive measures. A total number of 518 diarrheic sheep and goats belonging to 7 mixed flocks of sheep and goats were enrolled and from which rectal swabs were collected and subjected to culture for Salmonella spp. Enterotoxin (stn) gene was detected using polymerase chain reaction assay (PCR), and the PCR amplicons of nine randomly selected strains were purified, sequenced and deposited in the GenBank. The …
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