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Activation of NF-κB-dependent antiapoptotic genes may factor in the chemoresistance of pancreatic cancer. It is not known whether NF-κB subunit composition changes during oncogenesis and regulates overall NF-κB activation. We compared the relative expression of NF-κB subunits with nuclear activation of p65 between variably differentiated pancreatic cancer cells.

Proliferating human pancreatic cancer cells (PANC-1, BxPC-3) and nonmalignant intestinal cells (FHS 74 Int) were harvested. Baseline expression of NF-κB subunits (p65, p52, p50, c-Rel) and its inhibitor IκB-α were determined by Western blot. Nuclear NF-κB p65 activity was measured by ELISA. Results were analyzed by ANOVA (P < 0.05) and Tukey’s HSD for pairwise comparisons when appropriate (P < 0.05).

Constitutive expression of NF-κB subunits was detected in proliferating …