作者
Y Basaki, F Hosoi, Y Oda, A Fotovati, Y Maruyama, S Oie, M Ono, H Izumi, K Kohno, K Sakai, T Shimoyama, K Nishio, M Kuwano
发表日期
2007/4
期刊
Oncogene
卷号
26
期号
19
页码范围
2736-2746
出版商
Nature Publishing Group
简介
Y-box-binding protein 1 (YB-1), which is a member of the DNA-binding protein family containing a cold-shock domain, has pleiotropic functions in response to various environmental stimuli. As we previously showed that YB-1 is a global marker of multidrug resistance in ovarian cancer and other tumor types. To identify YB-1-regulated genes in ovarian cancers, we investigated the expression profile of YB-1 small-interfering RNA (siRNA)-transfected ovarian cancer cells using a high-density oligonucleotide array. YB-1 knockdown by siRNA upregulated 344 genes, including MDR1, thymidylate synthetase, S100 calcium binding protein and cyclin B, and downregulated 534 genes, including CXCR4, N-myc downstream regulated gene 1, E-cadherin and phospholipase C. Exogenous serum addition stimulated YB-1 translocation from the cytoplasm to the nucleus, and treatment with Akt inhibitors as well as Akt siRNA …
引用总数
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Y Basaki, F Hosoi, Y Oda, A Fotovati, Y Maruyama… - Oncogene, 2007