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Gene fusion is among the primary processes that generate new genes and has been well characterized as potent pathway of oncogenesis. Here, by high-throughput RNA sequencing in nine paired human endometrial carcinoma (EC) and matched non-cancerous tissues, we obtained that chimeric translin-associated factor X–disrupted-in-schizophrenia 1 (TSNAX–DISC1) occurred significantly upregulated in multiple EC samples. Experimental investigation showed that TSNAX–DISC1 appears to be formed by splicing without chromosomal rearrangement. The chimera expression inversely correlated with the binding of CCCTC-binding factor (CTCF) to the insulators. Subsequent investigations indicate that long intergenic non-coding RNA lincRNA-NR_034037, separating TSNAX from DISC1, regulates TSNAX–DISC1 production and TSNAX/DISC1 expression levels by extricating CTCF from insulators …