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Present investigation was conducted to screen selected genotypes of Cotton (G. hirsutum) for Jassids (A. biguttula biguttula) tolerance. Out of fifteen G. hirsutum stabilized genotypes screened for Jassids tolerance, none of the genotypes were completely resistant. However, two entries DHS-9 and DHS-62 were found to be medium resistant with grade 2. The identified medium resistant genotypes can be utilized in crossing programmes involving high yielding genotypes as parents to develop jassid resistant high yielding hybrids in cotton.