作者
Tracey E Swingler, Guy Wheeler, Virginia Carmont, Hannah R Elliott, Matthew J Barter, Muhammad Abu‐Elmagd, Simon T Donell, Raymond P Boot‐Handford, Mohammad K Hajihosseini, Andrea Münsterberg, Tamas Dalmay, David A Young, Ian M Clark
发表日期
2012/6
期刊
Arthritis & Rheumatism
卷号
64
期号
6
页码范围
1909-1919
出版商
Wiley Subscription Services, Inc., A Wiley Company
简介
Objective
To use an in vitro model of chondrogenesis to identify microRNAs (miRNAs) with a functional role in cartilage homeostasis.
Methods
The expression of miRNAs was measured in the ATDC5 cell model of chondrogenesis using microarray and was verified using quantitative reverse transcription–polymerase chain reaction. MicroRNA expression was localized by in situ hybridization. Predicted miRNA target genes were validated using 3′‐untranslated region‐Luc reporter plasmids containing either wild‐type sequences or mutants of the miRNA target sequence. Signaling through the Smad pathway was measured using a (CAGA)12‐Luc reporter.
Results
The expression of several miRNAs was regulated during chondrogenesis. These included 39 miRNAs that are coexpressed with miRNA‐140 (miR‐140), which is known to be involved in cartilage homeostasis and osteoarthritis (OA). Of these miRNAs …
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TE Swingler, G Wheeler, V Carmont, HR Elliott… - Arthritis & Rheumatism, 2012
