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To define for the first time the transcriptomes of normal and end-stage osteoarthritis (OA) hip cartilage.

RNA was isolated from cartilage within 2h of joint replacement surgery. Gene expression was analyzed using Agilent GeneSpring GX 11 following hybridization to Illumina Human HT-12 V3 microarrays. Real-time reverse-transcription polymerase chain reaction (RT-PCR) was used to validate the expression of six genes identified by microarray as differentially expressed. Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA) were used to investigate enriched functions or canonical pathways amongst differentially expressed genes respectively.

In total we identified 998 differentially expressed genes (fold change≥±1.5, P-value≤0.01) between neck of femur fracture (NOF) (n=10) and OA hip (n=9) patient cartilage. These differentially …