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High prolificacy in Garole sheep is due to the effect of the Booroola fecundity gene (FecB) mutation. In the present study, Garole rams were crossed with non-prolific Malpura ewes with the aim to increase prolificacy into Garole×Malpura (GM) crosses. The FecB genotyping was carried out by Forced-Restriction Fragment Length Polymorphism PCR technique in Garole (n=64), GM crosses (n=53) and Malpura sheep (n=62). In 64 Garole samples genotyped, 55 were homozygous (FecB^BB), 8 heterozygous (FecB^B+) and 1 non-carrier (FecB⁺⁺). The results showed that most Garole sheep (∼98.0%) carried the FecB allele. Out of 53 GM samples, 36 were heterozygous, 14 non-carriers and 3 were homozygous. Fifteen GM ewes genotyped that had at least one record of twins or triplets, revealed 1 homozygous and 14 heterozygous and 9 GM ewes that never produced twins were all non-carriers of the FecB mutation …