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A method for the determination of glyphosate (Glyph) and its metabolite aminomethylphosphonic acid (AMPA) in environmental water was developed with the emphasis on the clean-up procedure. The organic compounds in the environmental water were extracted with dichloromethane and the sample was concentrated by rotary evaporation. The concentrated sample was then passed through a strong anion-exchange (SAX) cartridge with an additional 2 ml of resin in the hydroxide form packed above the SAX packing. Glyph and AMPA were eluted with citrate buffer at pH 5.00 and determined directly by high-performance liquid chromatography with a postcolumn reactor and fluorescence detector. The detection limit and average recovery for both components were <2 μg/l and >85%, respectively.