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Drought stress in plants has become one of the major abiotic stress that limits the growth and development of plants which also contributes to low yields. Biotechnology which has new and emerging techniques can be use to solve the problem of drought stress in plants. This review aimed at identifying drought stress tolerance in plants at different stages, how plants respond to drought stress using different methods and the application of different biotechnology methods to improve drought tolerance in plants. Some important parameters about drought stress in plants such as drought tolerance mechanisms, plants responses to drought stress, gene regulation for drought stress tolerance in plants, effects of drought stress at different stages of plant growth and biotechnology methods in developing drought tolerance in plants was reviewed. The use of biotechnology methods such as classical breeding, use of genetic manipulation, genes from resurrection plants and Protoplast fusion was discussed. Drought stress affects our plants seriously and it leads to wilts, reduction of yields and death of plants at different developmental stages. Plants have developed different mechanisms to respond to drought stress but these mechanisms