作者
Hyo-Eon Jin, Soon-Sun Hong, Min-Koo Choi, Han-Joo Maeng, Dae-Duk Kim, Suk-Jae Chung, Chang-Koo Shim
发表日期
2009/3
期刊
Pharmaceutical research
卷号
26
页码范围
549-559
出版商
Springer US
简介
Purpose
To investigate the effect of 17α-ethynylestradiol (EE)-induced cholestasis on the expression of organic cation transporters (Octs) in the liver and kidney, as well as the pharmacokinetics and pharmacodynamics of metformin in rats.
Methods
Octs mRNA and protein expression were determined. The pharmacokinetics and tissue uptake clearance of metformin were determined following iv administration (5 mg/kg). Uptake of metformin, glucagon-mediated glucose production, and AMP-activated protein kinase (AMPK) activation were measured in isolated hepatocytes. The effect of metformin (30 mg/kg) on blood glucose levels was tested using the iv glucose tolerance test (IVGTT).
Results
The mRNAs of hepatic Oct1, renal Oct1, and Oct2 were decreased by 71.1%, 37.6%, and 94.5%, respectively, by EE cholestasis …
学术搜索中的文章
HE Jin, SS Hong, MK Choi, HJ Maeng, DD Kim… - Pharmaceutical research, 2009